Appearances in Story
In the beginning of this game a group of villains including Riddler, Two Face, Mr. Freeze, Poison Ivy, and Clayface were attempting to perform thievery. After Robin and Batman manage to defeat Clayface, they discover that Riddler got away on Two Face's van. Batman attaches a tracker on the van and tracks the villains down with the help of Robin. The heroes follow the Riddler into Mr. Freeze's base and quickly defeat Mr.Freeze and continue to fight Poision Ivy who rescues Riddler. Batman and Robin defeat Poision Ivy and then defeat Two Face. The last battle is with Riddler, and the heroes at last manage to defeat him as well.
Later, Penguin along with Killer Croc, Man-Bat, Bane, and Catwoman, plotted to take over the city by using several penguin robots. When Batman and Robin reached the sewers they imprisoned Killer Croc. Later the duo challenged Man Bat, who they also defeated. The final battle is with the Penguin and Catwoman. After defeating them the duo moves on to defeat their last enemy Joker.
Joker along with Mad Hatter, Scarecrow, Killer Moth, and Harley Quinn planned to spread chaos. Batman and Robin reach the factory that Joker was operating in and defeats Mad Hatter, however Joker escapes on his helicopter. The news comes that Commissioner Gordon was kidnapped and the heroes rush to the scene to fight Harley Quinn. Once Harley Quinn was defeated Batman fought Joker, the last boss. Eventually the duo defeated Joker and the story ends.